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Job Requirements of Biologist:
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Employment Type:
Contractor
-
Location:
Boston, MA (Onsite)
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Biologist
Careers Integrated Resources Inc
Boston, MA (Onsite)
Contractor
Job Title: Biologist
Location: Boston, MA
Duration: 18 Months+ (Possibilities of Extension)
Shift: Standard shift
Pay range: $50 - $55/ hr. on W2
Required Experience: 0-3 years
Job Description:
Responsibilities:
Qualification/ Requirements:
Additional information:
#sczr2
Location: Boston, MA
Duration: 18 Months+ (Possibilities of Extension)
Shift: Standard shift
Pay range: $50 - $55/ hr. on W2
Required Experience: 0-3 years
Job Description:
- We are seeking a highly skilled, passionate, and motivated scientist to join our diabetes team.
Responsibilities:
- This position will play a key lab-based role in advancing iPSC stem cell engineering at Client and will contribute to the development of the next generation of islet cell therapies.
- Successful candidates will have experience with human iPSC cell culture, differentiation, banking, gene editing, molecular biology techniques, and assay development.
- This position will report to Director – Islet Cell Therapy, Stem Cell Biology and Cell Engineering.
- Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells.
- Routine iPSC cell culture, passaging, and media making.
- Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening, and delivery.
- Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows.
- Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR.
- Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments.
- Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance.
- Collaborate cross-functionally to support transfer of R&D assays to GMP.
- Maintain accurate and detailed records of all experiments, synthesize results, prepare reports, and present in meetings. Contribute to SOP development.
- Work seamlessly in executing workplans and meeting deadlines.
Qualification/ Requirements:
- BS/MS in Stem Cell Biology, Cell Biology, Bioengineering, or a related field of relevant industry experience in gene editing and cell therapy.
- 3+ years for MS
- 5+ years for BS
- Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques.
- Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting. Hands-on experience with cell delivery methods of gene editing tools.
- Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment.
- Strong organizational skills and diligent record keeping.
- Experience in pancreatic islet directed differentiation is highly desirable
- Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable.
Additional information:
- Position located in Seaport, Boston MA. This position has mandatory weekend cell culture responsibilities on rotation basis.
#sczr2
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