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Job Requirements of Research Assistant II:
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Employment Type:
Contractor
-
Location:
Cambridge, MA (Onsite)
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Research Assistant II
Careers Integrated Resources Inc
Cambridge, MA (Onsite)
Contractor
Job Title: Research Assistant II
Job Location: Cambridge, MA
Job Duration: 5-6 Months(possibility of extension)
Payrate: $39.00 - $42.00/ hr on w2
Duties and Responsibilities:
Job Location: Cambridge, MA
Job Duration: 5-6 Months(possibility of extension)
Payrate: $39.00 - $42.00/ hr on w2
Duties and Responsibilities:
- Support expression, conjugation, purification and characterization of proteins.
- Develop and conduct biophysical and functional assays to validate various protein designs.
- Perform plate-based assays to analyze samples downstream from in vivo studies.
- Analyze, integrate and interpret data from multiple sources.
- Diligently document work in digital systems and regularly communicate results in team and group settings.
- Prepare reagents, solutions, and samples for analysis, including samples collected from in vivo studies (e.g. tissue homogenization and protein extraction).
- Assist in method development, validation, and troubleshooting.
- Ensure compliance with safety regulations and good research practices.
- Work collaboratively in a team environment and manage priorities to achieve our goals.
- Bachelor’s or Master’s degree in Protein Biochemistry/Biochemistry preferred, or related field.
- With a Bachelor's Degree - At least 3-5 years of hands-on laboratory experience (industry).
- With a Master's Degree - At least 1–3 years of hands-on laboratory experience (industry).
- Experience with recombinant protein production, purification, and characterization.
- Experience in FPLC protein purification using AKTA systems and Unicorn software (e.g. affinity chromatography, SEC, and IEX).
- Experience in plate based functional assays (ELISA/client, functional assays, enzyme kinetics, chromogenic/fluorescent readouts, etc.)
- Experience with protein bioconjugation and conjugate purification.
- Experience with OligonucleoProduct and related methods a plus.
- Experience using Benchling (eLN).
- Protein characterization techniques (e.g. SDS-PAGE, Western Blots, endotoxin testing, HPLC, BLI (Octet) or SPR (Biacore)).
- Strong organizational and documentation skills.
- Ability to work independently and as part of a team.
- Excellent communication and problem-solving skills.
- A creative, problem-solving, collaborative, and goal-oriented mindset.
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