Biologist

Job Title: Biologist
Location: Boston, MA
Duration: 4 Months+

Pay Range: $49 - $50.75/hr. on W2

Note: 3-5 year’s Experience
 
Senior Scientist Immunology Responsibilities:
As a Sr. Scientist in Immunology, you will support the development of Client allogeneic cell therapies by developing immune cell-based assays, executing in vitro experiments, and assessing immune response to different gene edited cells. You will contribute to the earlystage validation of gene edits and will work closely with cross-functional teams to advance cell therapy programs to preclinical development.
 

• Develop and optimize cell-based assays using primary human immune cells, such as mixed lymphocyte reaction, T-cell, NK-cell cytotoxicity assays, phagocytosis assays, etc.
• Isolate and culture different immune cells from either PBMCs or fresh whole blood using immunomagnetic isolation techniques.
• Perform differentiation of monocyte to macrophages or dendritic cells, as needed
• Assess immune checkpoint receptor expression using multi-parameter flow cytometry techniques
• Use different gene editing techniques, including CRISPR to create knockouts or transgenic cell lines
• Gene edit different cell types including iPSCs, primary cells, and immortalized cell lines
• Use different methods, including cell sorting, single cell cloning to create gene edited clonal cell lines
• Design and execute experiments and analyze resulting data to evaluate efficacy of gene edited cells in in vitro assays
• Communicate experimental data effectively through written reports and oral presentations.
• Demonstrate flexibility and motivation to learn and apply new technologies for biological assays.

 
Basic Requirements:

• Bachelor’s degree in Immunology, Cell Biology, or a related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology.
• Master’s degree in Immunology, Cell Biology, or a related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology.

 
Additional Skills/Preferences:

• Prior experience with using live cell analysis platforms such as Incucyte and Xcelligence will be a plus
• Proficient in developing in vitro assays, including work with human cell lines, PBMCs, and whole blood assays.
• Proven ability to utilize immune cell and cytokine analytical techniques, including multiplex ELISA, MSD and flow cytometry

 
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